2017.10.19; DNA breakage underlies both learning, age-related damage | MIT News

  • Early-response genes, which are important for synaptic plasticity, are "switched off" under basal conditions by topological constraints. Neuronal activity triggers DNA breaks in a subset of early-response genes, which overrides these topological constraints, and "switches on" gene expression. Shown here is the topological constraint to early-response genes represented as an open switch (left) that is tethered by intact DNA. Formation of the break severs the constraint, and promotes the circuit to be closed (right). The "brain bulb" represents the manifestation of neuronal activity.

    Courtesy of the researchers

  • Early-response genes, which are important for synaptic plasticity, are "switched off" under basal conditions by topological constraints. Neuronal activity triggers DNA breaks in a subset of early-response genes, which overrides these topological constraints, and "switches on" gene expression. Shown here is the topological constraint to early-response genes represented as an open switch (left) that is tethered by intact DNA. Formation of the break severs the constraint, and promotes the circuit to be closed (right). The "brain bulb" represents the manifestation of neuronal activity.

    Courtesy of the researchers

  • Early-response genes, which are important for synaptic plasticity, are "switched off" under basal conditions by topological constraints. Neuronal activity triggers DNA breaks in a subset of early-response genes, which overrides these topological constraints, and "switches on" gene expression. Shown here is the topological constraint to early-response genes represented as an open switch (left) that is tethered by intact DNA. Formation of the break severs the constraint, and promotes the circuit to be closed (right). The "brain bulb" represents the manifestation of neuronal activity.

    Courtesy of the researchers

DNA breakage underlies both learning, age-related damage

Process that allows brains to learn and remember also leads to degeneration with age.

Helen Knight | MIT News correspondent

June 4, 2015

The process that allows our brains to learn and generate new memories also leads to degeneration as we age, according to a new study by researchers at MIT.

The finding, reported in a paper published today in the journal Cell, could ultimately help researchers develop new approaches to preventing cognitive decline in disorders such as Alzheimer’s disease.

Each time we learn something new, our brain cells break their DNA, creating damage that the neurons must immediately repair, according to Li-Huei Tsai, the Picower Professor of Neuroscience and director of the Picower Institute for Learning and Memory at MIT.

This process is essential to learning and memory. "Cells physiologically break their DNA to allow certain important genes to be expressed," Tsai says. "In the case of neurons, they need to break their DNA to enable the expression of early response genes, which ultimately pave the way for the transcriptional program that supports learning and memory, and many other behaviors."

Slower DNA repair

However, as we age, our cells’ ability to repair this DNA damage weakens, leading to degeneration, Tsai says. "When we are young, our brains create DNA breaks as we learn new things, but our cells are absolutely on top of this and can quickly repair the damage to maintain the functionality of the system," Tsai says. "But during aging, and particularly with some genetic conditions, the efficiency of the DNA repair system is compromised, leading to the accumulation of damage, and in our view this could be very detrimental."

In previous research into Alzheimer’s disease in mice, the researchers found that even in the presymptomatic phase of the disorder, neurons in the hippocampal region of the brain contain a large number of DNA lesions, known as double strand breaks.

To determine how and why these double strand breaks are generated, and what genes are affected by them, the researchers began to investigate what would happen if they created such damage in neurons. They applied a toxic agent to the neurons known to induce double strand breaks, and then harvested the RNA from the cells for sequencing.

They discovered that of the 700 genes that showed changes as a result of this damage, the vast majority had reduced expression levels, as expected. Surprisingly though, 12 genes — known to be those that respond rapidly to neuronal stimulation, such as a new sensory experience — showed increased expression levels following the double strand breaks.

To determine whether these breaks occur naturally during neuronal stimulation, the researchers then treated the neurons with a substance that causes synapses to strengthen in a similar way to exposure to a new experience.

"Sure enough, we found that the treatment very rapidly increased the expression of those early response genes, but it also caused DNA double strand breaks," Tsai says.

The good with the bad

In further studies the researchers were able to confirm that an enzyme known as topoisomerase IIβ is responsible for the DNA breaks in response to stimulation, according to the paper’s lead author Ram Madabhushi, a postdoc in Tsai’s laboratory.

"When we knocked down this enzyme, we found that both double strand break formation and the expression of early response genes was reduced," Madabhushi says.

Finally, the researchers attempted to determine why the genes need such a drastic mechanism to allow them to be expressed. Using computational analysis, they studied the DNA sequences near these genes and discovered that they were enriched with a motif, or sequence pattern, for binding to a protein called CTCF. This "architectural" protein is known to create loops or bends in DNA.

In the early-response genes, the bends created by this protein act as a barrier that prevents different elements of DNA from interacting with each other — a crucial step in the genes’ expression.

The double strand breaks created by the cells allow them to collapse this barrier, and enable the early response genes to be expressed, Tsai says.

"Surprisingly then, even though conventional wisdom dictates that DNA lesions are very bad — as this ‘damage’ can be mutagenic and sometimes lead to cancer — it turns out that these breaks are part of the physiological function of the cell," Tsai says.

Previous research has shown that the expression of genes involved in learning and memory is reduced as people age. So the researchers now plan to carry out further studies to determine how the DNA repair system is altered with age, and how this compromises the ability of cells to cope with the continued production and repair of double strand breaks.

They also plan to investigate whether certain chemicals could enhance this DNA repair capacity.

The paper represents an important conceptual advance in our understanding of gene regulation, according to Bruce Yankner, a professor of genetics and neurology at Harvard Medical School who was not involved in the research.

"The work elegantly links DNA strand break formation by the enzyme topoisomerase IIβ to the temporal control of transcription, providing the most compelling evidence to date that this is a core transcriptional control mechanism," he says.  "I anticipate that this advance will have broad implications ranging from the basic biology of transcription to pathological mechanisms involved in diseases such as Alzheimer’s disease."


Comments

Slicer

June 5, 2015

"further studies to determine how the DNA repair system is altered with age" - This is critical. Either figure out what's gone wrong in the cells and fix it, or replace the cells themselves wholesale; the third option is watching your thoughts themselves crumble out from under you as you mutter incoherencies in a nursing home.

Lane Simonian

June 7, 2015

This is a very important finding as it elucidates one of the key pathways involved in Alzheimer's disease.

Activation of the NMDA receptor leads to synaptic plasticity and neurogenesis via the phosphatidylinositol-3 kinase/Akt pathway and to DNA damage via activation of caspases by peroxynitrites. The latter damage can be repaired by PARP under normal circumstances. However, when the production of peroxynitrites exceeds the ability of antioxidants to scavenge them, the neuroprotective phosphatidyinositol-3 kinase/Akt pathway becomes blocked and caspases cleave PARP so that DNA damage cannot be repaired. Unrepaired DNA damage and the subsequent mitochondrial failure and the death of neurons can occur without either amyloid or neurofibrillary tau tangles being present. Or put another way one can have Alzheimer's disease without having either amyloid or tau tangles.

Peroxynitrites also inhibit the release and synthesis of neurotransmitters involved in short-term memory, sleep, mood, social recognition and alertness, and reduce blood flow and the transport of glucose in the brain which can lead to apathy, wandering, and delusions.

The most powerful peroxynitrite scavengers such as eugenol in rosemary essential oil via aromatherapy and ferulic acid and syringic acid in panax ginseng have already partially reversed Alzheimer's disease in clinical trials.

http://onlinelibrary.wiley.com...

http://www.ncbi.nlm.nih.gov/pm...

Once oxidative stress is understood to be the primary driver of Alzheimer's disease, then the means to the effective treatment of the disease becomes possibel.

knute5

June 22, 2015

So how do I up my intake of topoisomerase IIβ? Is it in flax seeds? Kale?

Eric

June 22, 2015

If there is a link to this paper, It is not as obvious as it should be.

Tom Speed

June 24, 2015

Why was my comment removed? It was on topic.

When the truth won't do... :/

I tried.

google U.S. Patent 6630507 if you have loved ones with alzheimers/parkinsons or any other neurological condition.

curious guest

September 16, 2015

could it be related with Multiple Personality Disorder , when DNA recovery mechanism have problem